Release 3.0
last update on 10 Dez 2009
This Internet database integrates information on sequence and structure of lipases and related proteins sharing the same a/b hydrolase fold to facilitate protein engineering. We acknowledge your comments or contribution to this page: please send us information to be included in this database.
The updated LED (release 4.0.0) is accessible at
Browse database by classification

a/b hydrolase fold families

GGGX class   GX class   Y class

Browse database by organism


Browse database by structures


Perform homology search against the lipase database

BLAST search
Query the Lipase Engineering Database using SQL

SQL query
Download database content

Database content
Links to related websites

This database is maintained by Jürgen Pleiss. Institute of Technical Biochemistry, University of Stuttgart, Allmandring 31, 70569 Stuttgart, Germany


Widmann M., Trodler P. & Pleiss J. (2010). The isoelectric region of proteins: asystematic analysis. PLoS One5:e10546-e10546

Widmann M., Juhl PB. & Pleiss J. (2010). Structural classification by the Lipase Engineering Database: a case study of Candida antarctica lipase A. BMCGenomics 11: 123

Widmann M., Clairo M., Dippon J. & Pleiss J. (2008). Analysis of the distribution of functionally relevant rare codons. BMC Genomics 9: 207

Koschorreck M., Fischer M., Barth S. & Pleiss J. (2005). How to find soluble proteins: a comprehensive analysis of alpha/beta hydrolases for recombinant expression in E. coli. BMC Genomics6: 1-10

Barth S., Fischer M., Schmid R.D. & Pleiss J. (2004). The database of epoxide hydrolases and haloalkane dehalogenases: one structure., manyfunctions. Bioinformatics 20: 2845-2847

Barth S., Fischer M., Schmid R.D. & Pleiss J. (2004). Sequence and structure of epoxide hydrolases: a systematic analysis. Proteins 55: 846-855

Fischer M. & Pleiss J. (2003). The Lipase Engineering Database: a navigation and analysis tool for protein families. Nucleic Acids Res 31: 319-321

Pleiss J., Fischer M., Peiker M., Thiele C. & Schmid R.D. (2000). Lipase Engineering Database: understanding and exploiting sequence-structure-function relationships. J Mol Catal B: Enzym 10: 491 -508

Pleiss J., Scheib H., Schmid R.D. (2000). The his gap motif in microbial lipases: a determinant of stereoselectivity toward triacylglycerols and analogs. Biochimie 82: 1043-1052

Pleiss J., Fischer M., Schmid R.D. (1998). Anatomy of lipase binding sites: the scissile fatty acid binding site. Chem Phys Lipids 93: 67-80

This database system was developed by Markus Fischer. We acknowledge valuable contributions by Sandra Barth, Claudia Schmidt-Dannert, Dang Sy Hai, Melanie Grieb, Christoph Kaiser, Michael Knoll, Thomas Kosian, Marcus Peiker, Darius Schwenger, Alexander Steudle, Marco Strohmeier, Claudia Thiele, Sadhna Tyagi, Thai Ke Quan, Florian Wagner, Michael Widmann, Peter Benjamin Juhl and Xiaolei Yu.